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CH Instruments
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CH Instruments
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Image Search Results
Journal: The Journal of Neuroscience
Article Title: Cannabinoids Promote Oligodendrocyte Progenitor Survival: Involvement of Cannabinoid Receptors and Phosphatidylinositol-3 Kinase/Akt Signaling
doi: 10.1523/JNEUROSCI.22-22-09742.2002
Figure Lengend Snippet: Inhibition of PI3K results in the blockade of cannabinoid-induced phosphorylation of Akt and GSK-3β.A, Whole-cell lysates were prepared and immunoblotted as described in Materials and Methods with antibodies that recognize phospho-Akt (Ser473), phospho-GSK-3β (Ser9), and total Akt and GSK-3β.B, Oligodendrocyte progenitors were treated for 30 min with either 100 nm wortmannin or 10 μmLY294002 and then stimulated for 10 min with the nonselective cannabinoids HU210 (500 nm) and (+)-Win 55,212-2 (25 nm). The densitometric data represent the means ± SEM of three independent experiments performed in duplicate. *p < 0.001 versus control untreated cells; #p < 0.001 versus HU210- or (+)-Win 55,212-2-stimulated cultures.
Article Snippet: The cannabinoids (+)Win 55,212-2 and arachidonyl-2′-chloroethylamide/(all Z)- N -(2-cycloethyl)-5,8,11,14-eicosatetraenamide (ACEA) and the PI3K inhibitors {"type":"entrez-nucleotide","attrs":{"text":"LY294002","term_id":"1257998346","term_text":"LY294002"}} LY294002 and
Techniques: Inhibition